Dissecting super-enhancer hierarchy based on chromatin interactions
Dissecting super-enhancer hierarchy based on chromatin interactions
Abstract Recent studies have highlighted super-enhancers (SEs) as important regulatory elements for gene expression, but their intrinsic properties remain incompletely characterized. Through an integrative analysis of Hi-C and ChIP-seq data, we find that a significant fraction of SEs are hierarchically organized, containing both hub and non-hub enhancers. Hub enhancers share similar histone marks with non-hub enhancers, but are distinctly associated with cohesin and CTCF binding sites and disease-associated genetic variants. Genetic ablation of hub enhancers results in profound defects in gene activation and local chromatin landscape. As such, hub enhancers are the major constituents responsible for SE functional and structural organization.
Huang Jialiang、Li Kailong、Orkin Stuart H.、Xu Jian、Liu Xin、Cai Wenqing、Yuan Guo-Cheng、Zhang Yuannyu
Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard T.H. Chan School of Public Health||Division of Hematology/Oncology, Boston Children?ˉs Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical SchoolChildren?ˉs Medical Center Research Institute, Department of Pediatrics, University of Texas Southwestern Medical CenterDivision of Hematology/Oncology, Boston Children?ˉs Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical School||Howard Hughes Medical InstituteChildren?ˉs Medical Center Research Institute, Department of Pediatrics, University of Texas Southwestern Medical CenterChildren?ˉs Medical Center Research Institute, Department of Pediatrics, University of Texas Southwestern Medical CenterDivision of Hematology/Oncology, Boston Children?ˉs Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Stem Cell Institute, Harvard Medical SchoolDepartment of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard T.H. Chan School of Public HealthChildren?ˉs Medical Center Research Institute, Department of Pediatrics, University of Texas Southwestern Medical Center
生物科学理论、生物科学方法生物科学研究方法、生物科学研究技术分子生物学
Super-enhancerChromatin interactionHub enhancerHierarchyCTCF
Huang Jialiang,Li Kailong,Orkin Stuart H.,Xu Jian,Liu Xin,Cai Wenqing,Yuan Guo-Cheng,Zhang Yuannyu.Dissecting super-enhancer hierarchy based on chromatin interactions[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/149583.点此复制
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