MicroRNA let-7i转染诱导的CD86-DCs对Treg作用的研究

Objective Too further research through which DC subsets did miRNAlet-7i play a role in immune regulation and the impact of this sub-group on Treg. Methods miRNAlet-7i inhibitor transfected DC were divided into CD86+DCs and CD86-DCs using immunomagnetic beads, cocultured with T cells and observed the effect of CD86+DC and CD86-DC on T cell proliferation、the number of Treg and IL-10 secretion. The expression of SOCS1 of different cell subsets which is the target of miRNAlet-7i is detected by Western blotting. Results Transfecting with miRNAlet-7i inhibitor significantly impeded DC surface expression of CD80 and CD86 and the secretion of pro-inflammatory cytokine. There were two subpopulations of LPS-stimulated DCs with downregulated miRNAlet-7i, CD86+DC and CD86-DC, and it was the CD86-DCs that were more effective in inducing T cell hyporesponsiveness and the secretion of anti-inflammatory cytokine,and inhibiting secretion of pro-inflammatory cytokine.CD4+CD25+Tregs, isolated from CD86-DCs and T cells cocultured medium, can inhibition of T cell proliferation more effective and promote inhibitory cytokine IL-10secretion.And miRNAlet-7i inhibitors primary increase the expression of SOCS1 in CD86-DC. Conclusion Through acting on SOCS1of CD86-DC that miRNAlet-7i play a role in the regulation of Treg.