采用SSR和SRAP分子标记构建葡萄分子遗传图谱

n F1 population was created by the cross '87-1'×'9-22'. The female parent 87-1 was an extremely early maturing cultivar with strong flavor. The male parent was an excellent breeding line producing large berries maturing late. 149 individuals were randomly chosen as the mapping population. Molecular genetic map for each parent as well as the consensus map were constructed using simple sequence repeat(SSR)and Sequence-Related Amplified Polymorphism(SRAP)marker by software JoinMap 3.0. The 87-1 map covers a total length of 1272.9 cM distributed in 21 linkage groups, and consists of 163 molecular markers with an average distance between adjacent markers of 8.9 cM. The 9-22 map covers a total length of 1267.4 cM distributed in 20 linkage groups, and consists of 158 molecular markers with an average distance between adjacent markers of 9.1 cM. The consensus map covers a total length of 1537.1 cM distributed in 21 linkage groups and one doublet, and consists of 217 molecular markers with an average distance between adjacent markers of 7.8 cM. The length of linkage group is 69.8 cM on average. The maps has covered the 19 chromosomes of Vitis genome, and can lay a solid foundation for further studies such as QTL mapping of correlated traits and marker assisted selection.