姜黄素对鬼臼毒素细胞毒性的保护作用

Objective To demonstrate the protective effect and mechanisms of curcumin administrastion against the cytotoxicity of podophyllotoxin. Methods Protective effects of curcumin against podophyllotoxin poisoning were examined in VERO cells. Cell toxicity was measured by MTT assay. Cell cycle and membrane potential were determined by flow cytometry. Microtubules were observed by confocal laser scanning microscope. Results Curcumin could attenuate podophyllotoxin-induced toxicity in VERO cells, recover podophyllotoxin-induced G2/M arrest, membrane potential change and the disturbance of microtubule structures. Conclusion Curcumin had protective effects against the podophyllotoxin induced cytotoxicity. The mechanisms might be associated with the competitive binding to tubulin.