JZTX-IX对Kv2.1通道的作用机制研究
Molecular determinants for the tarantula toxin JZTX-IX interacting with potassium channel subtype Kv2.1
JZTX-IX 是一种从敬钊缨毛蛛毒液中分离纯化的35个氨基酸残基的多肽。JZTX-IX能抑制电压门控钾通道Kv2.1电流，而且能减小通道去激活尾电流的时间常数，加速通道从激活到关闭状态的转变速率，被认为是一种门控修饰毒素。根据毒素的同源性推测与JZTX-IX作用的受体部位是Kv2.1通道上电压传感器的S3b-S4浆叶结构（VS），但是Kv2.1通道该结构哪些氨基酸残基是与JZTX-IX作用的关键残基还不清楚。本研究对Kv2.1通道电压传感器的S3b-S4桨叶结构的21个氨基酸残基进行单突变，分别检测 JZTX-IX对该通道突变体的作用，结果表明Kv2.1通道上S3b-S4浆叶结构的I273、F274 和E277是关键活性残基。
JZTX-IX is a 35 amino acid residues polypeptide, purified from the venom of Tarantula Chilobrachys jingzhao. JZTX-IX inhibited the voltage-gated potassium channel Kv2.1 current, reduced the time constant of deactivation tail current and speeded the channel transformation rate of state from activation to close,was considered to be the gating modifier toxins. The previous results showed that the receptor site of the Kv2.1 channels to JZTX-IX was S3b-S4 blade structure on voltage sensor, whereas the key residues did not unknown. In this study, the effects of JZTX-IX to 21 single mutations on S3b-S4 blade structure were detected and the results showed that the three amino acids I273, F274 and E277 on the S3b-S4 blade structure was the key active residues on Kv2.1 channel.
王美迟、吴远远、陶怀、邓梅春
基础医学分子生物学
蛋白质钾通道电压传感器门控修饰毒素
Protein Potassium channelvoltage sensorgating modifier toxins
王美迟,吴远远,陶怀,邓梅春.JZTX-IX对Kv2.1通道的作用机制研究[EB/OL].(2013-07-09)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201307-134.点此复制
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