复方浙贝颗粒联合阿霉素影响K562/A02移植瘤细胞耐药相关酶表达研究

Objective：To investigate the drug-combination of Compound Zhe Bei Granule (CZBG) and doxorubicin effects on the expression of GST and Topo-II in K562/A02 cell line multidrug resistance tumor xenografts in mice. Method：Tumor xenografts model were established by injecting the multidrug resistance cell line K562/A02 in the axillary flank of BALB/c-nu-nu mice. Drug-comgbination of CZBG intragastric administration and doxorubicin intraperitoneal injection (i.p.) were given to the BALB/c-nu nude mice. The tumor xenografts were made into slice after the dissection, and the expression ofGST and Topo-II in K562/A02 tumor xenografts in mice were investigated in immunohistochemical techniques. the integral optical density (IOD) of GST and Topo-II in K562/A02 tumor xenografts were measured by Image ProPlus 6.0. Results：Compared with the single treatment of doxorubicin i.p group., the groups of the combination of the doxorubicin and CZBG with dosage classified by three types(high, middle, low) can decrease IOD of GSH and Topo-II in K562/A02 tumor xenografts with statistical significance（p<0.05）.Conclusion：The combination of CZBG and doxorubicin can decrease the expression of GST and Topo-II in K562/A02 multidrug resistance tumor xenografts in mice.