蜡梅一个MYB转录因子功能的初步分析

Wintersweet (Chimonanthus praecox) is a traditional ornamental shrub in China. The inner-tepals of its flowers presented different colors with the different cultivars. Two genotypes -- 'H29'with mauve inner tepals and 'H64'with yellow inner tepals, were sampled in this study. Then from the flowers of two genotypes,two transcriptomic libraries were separately constructed using RNA-seq, and from the libararies a MYB gene--CpMYB216585 was indentified and cloned. The full-length cDNA of CpMYB216585 was 756 bp which codes for 252 amino acids. By analysis of its sequences, it indicated that the gene is a transcription factor with inhibiting effect. Besides, through phylogenetic treeing of some MYBs and analysis of the gene expression profiles in different flowering time we can inferred that the CpMYB21658 possibly acts as a negative regulator to biosynthesis of flavonoids. Furthermore the promotor of this gene contains some photoresponsive and hormones responsive elements.