水溶性低代聚酰胺胺－金纳米复合物的制备、光学性质和细胞染色实验研究
Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites
在2.0代胺末端聚酰胺胺（NH2-PAMAM G2.0）的水溶液中，通过紫外可见分光光谱和荧光分光光谱研究了Au(III)/PAMAM不同配比条件下，NH2-PAMAM G2.0-Au复合物形成过程的溶液化学行为和光学特征。制备的NH2-PAMAM G2.0-Au复合物为NH2-PAMAM包裹Au纳米粒子的外包型纳米复合物，分布均匀，在冷冻时十分稳定, 水溶性和生物相容性好，呈现较强的蓝紫色荧光发射。复合物中金纳米粒子为2.5nm左右，并随着溶液中PAMAM浓度增大而趋小。PAMAM在复合物形成前起骨架分散和稳定作用，在复合物形成后起介质分散和稳定的作用。初步的人胚肺成纤维(HELF)细胞系染色试验表明该复合物纳米粒子可望作为生物分析和医学诊断用光学造影剂。
he solution chemical and optical characteristics of formation of NH2-PAMAM G2.0-Au nanocomposites in the aqueous solution of amine-terminated polyamidoamine dendrimer G2.0 (NH2-PAMAM G2.0) under various mole ratios of Au(III)/PAMAM were studied by both UV-Vis spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle was surrounded by several NH2-PAMAM dendrimers, emitted strong bluish violet fluorescence, and were uniform, water soluble and biocompatible as well as very stable in frozen conditions. The sizes of gold nanoparticles in the nanocomposites were about 2.5nm and decreased with increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2.0 played an important role in being as host or micro-reactor for Au(Ⅲ) before Au(Ⅲ) reduction and being as dispersant and stabilizer for gold nanoparticles after Au(Ⅲ) reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast (HELF) cell lines s
傅金殿、申丽、聂珍媛、夏金兰
生物科学研究方法、生物科学研究技术生物化学生物物理学
聚酰胺胺 树枝状大分子 纳米复合物 金纳米粒子 光学性质 细胞染色
polyamidoamine dendrimer nanocomposites gold nanoparticles optical properties cell staining
傅金殿,申丽,聂珍媛,夏金兰.水溶性低代聚酰胺胺－金纳米复合物的制备、光学性质和细胞染色实验研究[EB/OL].(2005-04-27)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/200504-174.点此复制
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