Nasopharyngeal Panbio COVID-19 antigen performed at point-of-care has a high sensitivity in symptomatic and asymptomatic patients with higher risk for transmission and older age
Nasopharyngeal Panbio COVID-19 antigen performed at point-of-care has a high sensitivity in symptomatic and asymptomatic patients with higher risk for transmission and older age
ABSTRACT BackgroundPerformance of point-of-care tests in clinical practice remains undetermined. We aimed to evaluate the performance of the nasopharyngeal Panbio COVID-19 antigen Rapid Test Device in real-life conditions in different clinical scenarios. MethodProspective study conducted in three primary care centers (PCC) and an emergency department. The antigen test was performed at point-of-care in nasopharyngeal and nasal swabs, and in saliva. Positive and negative percent agreement (PPA, NPA) were calculated with the RT-PCR assay as reference standard. ResultsOf 913 patients included, 296 (32.3%) were asymptomatic and 690 (75.6%) came from the PCC. Nasopharyngeal swabs were collected from 913, nasal swabs from 659, and saliva from 611 patients. RT-PCR was positive in 196 (21.5%) nasopharyngeal samples (NPS). Overall PPA (95% CI) in NPS was 60.5% (53.3-67.4), and it was lower in nasal swabs (44.7%) and saliva (23.1%). Test performance in NPS was largely dependent on the cycle threshold (Ct) in RT-PCR, with PPA>90% for Ct≤25 and ≥80% for Ct<30. In symptomatic patients, the PPA was 95% for Ct≤25; ≥85% for Ct<30, and 89% for the symptom triad of fever, cough and malaise. Performance was also dependent on age, with PPA of 100% in symptomatic patients >50 years with Ct<25. In asymptomatic patients, the PPA was 86% for Ct<25. In all cases, NPA was 100%. ConclusionThe nasopharyngeal Panbio COVID-19 antigen test performed at point-of-care is highly sensitive in symptomatic patients, particularly with Ct<30 and older age. The test was useful to identify asymptomatic patients with lower Ct values and therefore with contagious risk. Key pointsThe nasopharyngeal Panbio-COVID-19 antigen test performed in real-life conditions at point-of-care is highly sensitive in symptomatic patients, particularly with Ct<30 and older age. The test is useful to identify asymptomatic patients with lower Ct values and therefore with contagious risk.
Gonzalo Nieves、Guti¨|rrez Jos¨| Antonio、Garc¨aa Jos¨| Alberto、Candela Inmaculada、S¨￠nchez Manuel、Masi¨￠ Mar、de Gregorio Carlos、Guti¨|rrez F¨|lix、Fern¨￠ndez-Gonz¨￠lez Marta、Guijarro Jorge、Agull¨? Vanesa、de la Tabla Victoria Ortiz、Carvajal Mar
Microbiology Service, Hospital General Universitario de ElcheEmergency Department, Hospital General Universitario de ElcheInfectious Diseases Unit, Hospital General Universitario de ElcheSanta Pola Primary Care CenterDepartment 20th Health Authority, Conseller¨aa de Sanitat, Generalitat ValencianaInfectious Diseases Unit, Hospital General Universitario de Elche||Clinical Medicine Department, Universidad Miguel Hern¨￠ndezDepartment 20th Health Authority, Conseller¨aa de Sanitat, Generalitat ValencianaInfectious Diseases Unit, Hospital General Universitario de Elche||Clinical Medicine Department, Universidad Miguel Hern¨￠ndezInfectious Diseases Unit, Hospital General Universitario de ElcheAltabix Primary Care CenterInfectious Diseases Unit, Hospital General Universitario de ElcheMicrobiology Service, Hospital Universitario de San JuanInfectious Diseases Unit, Hospital General Universitario de Elche
医学研究方法临床医学耳鼻咽喉科学
COVID-19antigenPanbioSARS-CoV-2point-of-care
Gonzalo Nieves,Guti¨|rrez Jos¨| Antonio,Garc¨aa Jos¨| Alberto,Candela Inmaculada,S¨￠nchez Manuel,Masi¨￠ Mar,de Gregorio Carlos,Guti¨|rrez F¨|lix,Fern¨￠ndez-Gonz¨￠lez Marta,Guijarro Jorge,Agull¨? Vanesa,de la Tabla Victoria Ortiz,Carvajal Mar.Nasopharyngeal Panbio COVID-19 antigen performed at point-of-care has a high sensitivity in symptomatic and asymptomatic patients with higher risk for transmission and older age[EB/OL].(2025-03-28)[2025-07-09].https://www.medrxiv.org/content/10.1101/2020.11.16.20230003.点此复制
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