Temperature-induced changes in wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms
Temperature-induced changes in wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms
ABSTRACT Wheat (Triticum ssp.) is one of the most important human food sources. However, this crop is very sensitive to temperature changes. Specifically, processes during wheat leaf, flower and seed development and photosynthesis, which all contribute to the yield of this crop, are affected by high temperature. While this has to some extent been investigated on physiological, developmental and molecular levels, very little is known about early signalling events associated with an increase in temperature. Phosphorylation-mediated signalling mechanisms, which are quick and dynamic, are associated with plant growth and development, also under abiotic stress conditions. Therefore, we probed the impact of a short-term increase in temperature on the wheat leaf and spikelet phosphoproteome. The resulting data set provides the scientific community with a first large-scale plant phosphoproteome under the control of higher ambient temperature, which will be valuable for future studies. Our analyses also revealed a core set of common proteins between leaf and spikelet, suggesting some level of conserved regulatory mechanisms. Furthermore, we observed temperature-regulated interconversion of phosphoforms, which likely impacts protein activity.
Verstraeten Inge、Gevaert Kris、Vu Lam Dai、Zhu Tingting、IWGSC、van de Cotte Brigitte、Smet Ive De
Ghent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyDepartment of Biochemistry, Ghent University||VIB-UGent Center for Medical BiotechnologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems Biology||Department of Biochemistry, Ghent University||VIB-UGent Center for Medical BiotechnologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems Biology||Department of Biochemistry, Ghent University||VIB-UGent Center for Medical BiotechnologyLee?ˉs SummitGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems BiologyGhent University, Department of Plant Biotechnology and Bioinformatics||VIB Center for Plant Systems Biology
农业科学研究植物学生物科学研究方法、生物科学研究技术
WheatTemperaturePhosphorylationSignallingLeafSpikeletPhosphoproteomics
Verstraeten Inge,Gevaert Kris,Vu Lam Dai,Zhu Tingting,IWGSC,van de Cotte Brigitte,Smet Ive De.Temperature-induced changes in wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms[EB/OL].(2025-03-28)[2025-04-28].https://www.biorxiv.org/content/10.1101/261065.点此复制
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