棉纤维特异启动子LTP12驱动的基因phaB、phaC双价载体构建及其原核表达研究

he poly-3-hydroxybutyrate(PHB) have anti-crease and warmth retention , However, the anti-crease and warmth retention of the cottonfiber would improved if it has PHB in the cotton fiber , So the PHB synthase genes was introduced cotton by Genetic Engineering Technology that the PHB was synthased in the cotton fiber, the waterproofness、 anti-crease and warmth retention of the cotton fiber was improved. In this work, promoter LTP12 for cotton fiber and genes phaB and phaC are cloned. then their plant expression Vector pBLTPPBPC drived by Specific Promoter LTP12 was constructed and the two genes were induced prokaryotic expression in Escherichia coli BL21. The expression products of the genes phaB and phaC was detected by polyacrylamide gel electrophoresis. The result showed that gel electrophoresis of gene phaB has a line of molecular weight about 35KD, the molecular weight of the gene phaB is the same size of 35KD, however, there is no special line on the gel electrophoresis of gene phaC, which may be connected with gene phaC.