冠心病患者染色体9p21区域rs2383207位点遗传变异与相邻基因表达水平的关系
ssociation between genetic variation of rs2383107 in 9p21 region and expression levels of the adjacent genes in patients with coronary heart disease
目的：探讨中国汉族冠心病患者染色体9p21区域rs2383207位点遗传变异与相邻基因CDKN2A和CDKN2B表达水平的关系。方法：选择冠心病患者91例为研究对象。采用Sequenom MassARRAY飞行时间质谱法对rs2383207位点进行基因分型，采用实时荧光定量PCR检测CDKN2A和CDKN2B基因的表达水平，采用SPSS16.0进行统计分析。结果：染色体9p21区域rs2383207位点不同基因型冠心病患者外周血中CDKN2A表达量差异显著（P＜0.05），而CDKN2B基因的表达量无显著差异（P＞0.05）。结论：rs2383207位点遗传变异可能是通过影响CDKN2A基因的表达影响冠心病的发生。
Objective: To explore the relationship between rs2383207 genetic variation in 9p21 region and expression levels of the adjacent genes in patients with coronary heart disease (CHD) in Chinese Han Population. Methods: We recruited 91 patients with CHD as subjects . Rs2383207 loci were genotyped with Sequenom MassARRAY time of flight mass spectrometer (TOF); gene expression levels of CDKN2A and CDKN2B were detected with Real-time Quantitative PCR. SPSS16.0 was used for result analysis. Results: Gene expression level of CDKN2A was different in peripheral blood of CHD patients with different genotype (P＜0.05), while CDKN2B was not (P＞0.05). Conclusions: Genetic variation of rs2383207 locus influencing the accurrence of CHD may be by influencing the expression level of CDKN2A gene.
吴娟、叶琳、王树越、周丽婷、朱健、陈新、郑东春
基础医学遗传学内科学
染色体9p21冠心病遗传变异基因表达
hromosome 9p21Coronary heart diseaseGenetic variantsgene expression
吴娟,叶琳,王树越,周丽婷,朱健,陈新,郑东春.冠心病患者染色体9p21区域rs2383207位点遗传变异与相邻基因表达水平的关系[EB/OL].(2013-12-31)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201312-1296.点此复制
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