平滑肌细胞在壳聚糖-明胶三维支架中的生长

Pore architecture in 3D polymeric scaffolds is known to play a critical role in tissue engineering as it provides the vital framework for the seeded cells to organize into a functioning tissue. In this work, we fabricated chitosan-gelatin 3D scaffolds using freeze-dry technique. We got the porous scaffolds of different porosities and pore sizes through different freezing temperature regimes and different concentrations. From the scanning electron microscopic images, we found that the scaffold formed the largest diameter(60-100µm) when the scaffold was fabricated on concentration (1wt%) and pre-freezing temperature (-20℃). However, the scaffold formed highly interconnected pores with largest porosity (93%) when pre-freezing temperature was -196℃. The airway smooth muscle cells (third generation) separated from rat were seeded on the 3D scaffold. And the cell proliferation was evaluated by MTT assay in 6 days. The result indicated that cells proliferated well on the scaffolds. We also observed the cell growth by scanning electron microscopy and immunofluorescence staining. This study fabricated highly interconnected porous scaffolds for developing functional 3D chitosan-gelatin matrices for potential tissue engineering, biomedical and biotechnological applications.