柱前衍生RP-HPLC 法测定苍耳子和意大利苍耳子中氨基酸的种类

In this study, the types and content of amino acids in Xanthium sibiricum Patr. (XSP) and Xanthium italicum Moretti (XIM) were determined, and a precolumn derivatization RP-HPLC method was established. The free amino acids in XSP and XIM were ultrasonically extracted by 50% ethanol as solvent. The hydrolyzed amino acids were extracted by hydrolysis method in HCl (6 mol?L-1). Phenylisothiocyanate was selected as the precolumn derivatization agent to analyze the amino acids by gradient elution. The results indicate that twenty kinds of amino acids in XSP and XIM are characterized and they have good linearity in the range of 0.039 1～1.250 mmol?L-1 (R2≥ 0.999 3). The average recoveries are 92.70~98.44% and the relative standard deviation (RSD) values are between 0.70% and 3.27%. The method has good sensitivity, accuracy, repeatability and stability. Twenty kinds of amino acids are isolated from the XSP and XIM, and the extraction quality is calculated.