胆固醇基-普鲁兰自组装纳米粒的体外HepG2细胞摄取研究

im: To evaluate the influence factors and mechanism of uptake of cholesterol-modified pullulan nanoparticles by HepG2 cells. Methods: FITC was conjugated to CHSP, and FITC-CHSP nanoparticles were prepared by dialysis. Uptake of FITC-CHSP nanoparticles by HepG2 cells was quantified by fluorometry, to investigate the effect of nanoparticle concentration, incubation time and temperature on the uptake. Meanwhile, several selective endocytosis inhibitors, chlorpromazine, methyl-β-cyclodextrin and amiloride were selected to study the potential endocytosis mechanism. In vitro experiments with endocytic inhibitors suggested that clathrin-mediated endocytosis, and caveolae-mediated endocytosis were involved in the internalization of FITC-CHSP nanoparticles. Results: The uptake of FITC-CHSP nanoparticles by HepG2 cells at 37 oC increased with the incubation time over a 2 h period and had no significant further increase beyond 2 h. Furthermore, the uptake of nanoparticles by HepG2 cells was found increased with increase in the concentration of nanoparticles, but the efficiency of uptake was reduced at higher doses. Besides, there was a significant reduction in nanoparticles uptake at 4 C. Conclusion: The study provides experimental evidence for revelation of the endocytosis mechanism of hydrophobically modified polysaccharides self-assembled nanoparticles and medical security application.