茶树冷驯化过程中基因表达差异的初步分析
Preliminary Study on Gene Different Expression during Cold Acclimation in Tea Plant (Camellia sinensis)
采用mRNA差别显示的方法对茶树冷驯化过程中基因差异表达进行了初步研究，从58条差异片段得到稳定扩增的差异片段23条，用RT-PCR的方法鉴定其假阳性，得到5个阳性片段，其中3个片段在冷驯化过程中表达量增加，另外2个片段的表达量降低。经过BLAST比对分析，其中一个片段序列与高粱反式玉米素-O-葡糖基转移酶同源性较高；一个与黄瓜叶绿体合成相关蛋白基因同源性较高；一个为茶树核酮糖-1,5-双磷酸羧化酶/加氧酶小亚基基因片段；一个与细胞色素b亚基有较高同源性；还有两个片段序列比对没有同源序列，可能为新基因。
ifferent Gene expression during cold acclimation were preliminary studied by mRNA differential display reverse transcription-PCR (DDRT-PCR) in tea plant (Camellia sinensis). 23 differentially expressed cDNA fragments were steadily re-amplified. Five fragments were confirmed by RT-PCR analysis. Among them, 3 were increasing while other two were decreasing during the cold acclimation process. Sequencing of three differential fragments revealed homology to putative cis-zeatin O glucosyl- transferase of Sorghum bicolor, chloroplast gene for chloroplast product of Cucumis sativus，ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS) mRNA of Camellia sinensis, respectively, while the other two showed no homologous to known genes.
杨亚军、梅菊芬、王新超、黎星辉
分子生物学植物学遗传学
茶树冷驯化基因表达差异特异片段
ea plant (Camellia sinensis) Cold acclimation mRNA differential display reverse transcription-PCR fragments
杨亚军,梅菊芬,王新超,黎星辉.茶树冷驯化过程中基因表达差异的初步分析[EB/OL].(2007-11-26)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/200711-516.点此复制
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