饮料中人工合成甜味剂超高效液相色谱的快速检测方法

n ultra performance liquid chromatographic method for simultaneous separation and determination of four artificial sweeteners (sodium saccharin, aspartame, acesulfame and neotame) in a single injection is proposed. The separation was performed by a gradient program and determination by ultraviolet absorbance detection at 220nm. Good linearities between the concentrations of all analytes and peak area responses were achieved over the range 0.5-20 mg/L. The method has been successfully applied to the determination of the four sweeteners in drinks and powdered tabletop sweeteners, and the average recovery for sample ranged from 70.5% to 95.2%, and the relative deviation is between 0.50% and 8.7%.