兔成骨细胞、破骨细胞培养与功能观察

In this study, rabbit’s osteoblasts and osteoclasts were harvested through the enzyme digestion method and bone-marrow-cells induction approach, respectively. Then cultured osteoblasts were assayed by alkaline phosphate (ALP) staining , mineralized function and osteoclasted were identified through tartrate-resistant acid phosphatase (TRAP) staining , bone resorption assay and F-actin ring observation. The results showed that osteoblasts were available with ALP positive and good mineralized ability and osteoclasts were obtainable with TRAP positive, fine bone resorption ability , obvious F-actin ring around cytoplasm. It is suggest that the target osteoblast and osteocalst for following experiments are available.