复方祛疤凝胶中三七3种皂苷含量测定方法的改进
Study on improving the determination method of 3 kinds of saponine of Panax notoginseng in removing scar gel compound
目的 改进高效液相色谱法测定复方祛疤凝胶中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的方法。方法 色谱条件：Diamonsil C18柱( 250 mm×4.6 mm，5 μm)，流动相为乙腈-水，梯度洗脱( 0～25 min，25%～40％乙腈)，柱温35℃，ELSD漂移管温度48℃，气体流速为3.0 L/min。结果 三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的回归方程分别为：logY=1.630 1logX +1.498，r = 0.999 9（0.416～2.496 μg）；logY = 1.653 2logX +1.672 9，r = 0.999 6（1.694～10.164 μg）；logY = 1.7138 logX +1.687 4，r = 0.999 7（1.552~9.312 μg）。结论 采用改进的测定方法简单、结果可靠，为复方祛疤凝胶中三七药材的质量控制提供了快速准确的测定方法。
objective: To improve the determination method of Notoginsenoside R1, Ginsenoside Rg1and Ginsenoside Rb1 in removing scar gel compound by HPLC. Method : Chromatographiccondition: Diamonsil C18 (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile-water, gradient elution (0～25 min, acetonitrile from 25%～40%); column temperature 35℃; ELSD drift tube temperature 48℃; gas velocity 3.0 L/min. Result: Regression equations of Notoginsenoside R1, Ginsenoside Rg1and Ginsenoside Rb1 are logY=1.630 1logX +1.498, r = 0.999 9（0.416～2.496 μg）; logY = 1.653 2logX +1.672 9, r = 0.999 6（1.694～10.164 μg）; logY = 1.7138 logX +1.687 4, r = 0.999 7（1.552~9.312 μg）respectively. Conclusion: The improved determination method is simple and reliable, and it can provide quick and accurate determination method for quality control of Panax notoginseng in removing scar gel compound.
陈海亭、吴圣琴、尹蓉莉、李东芬、王宝才、陈金玉
药学医学研究方法
三七HPLC-ELSD三七皂苷R1人参皂苷Rb1人参皂苷Rg1
Panax notoginsengHPLC-ELSDNotoginsenoside R1Ginsenoside Rb1Ginsenoside Rg1
陈海亭,吴圣琴,尹蓉莉,李东芬,王宝才,陈金玉.复方祛疤凝胶中三七3种皂苷含量测定方法的改进[EB/OL].(2010-11-22)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201011-486.点此复制
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