1α,25(OH)2D3在卵巢癌细胞SKOV-3中调控lncRNAs表达谱

Long non-coding RNAs (lncRNAs) have been reported to be involved in processes of tumor progression. 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3), the active form of vitamin D, has an anti-proliferative effect on tumor cells. Therefore, differentially expressed lncRNAs regulated by 1α,25(OH)2D3 may be associated with tumor progress. This study was aimed to investigate the lncRNAs profiling pattern regulated by 1α,25(OH)2D3 in ovarian cancer cell line SKOV-3 using microarray analysis. After 72 hours of 1α,25(OH)2D3 treatment, 139 lncRNAs and 105 mRNAs were significantly dysregulated. Five lncRNAs were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In functional analysis, dysregulated lncRNAs can participate in biological processes related to systemic lupus erythematosus, alcoholism, osteoclast differentiation and cancer signaling pathways, such as Wnt, TNF and TGF-β receptor signaling. The dysregulated lncRNAs were associated with their co-expressed genes and may be mainly regulated by these three transcription factors, TAF7, POU2F2 and KAT2A. Additionally, there were 17 lncRNAs co-expressed with genes VDR, CYP24A1 and CDH1, suggesting that these lncRNAs acted as the connection to associate vitamin D signaling with EMT. These data constitute the first report of lncRNA expression patterns regulated by 1α,25(OH)2D3 in human ovarian cancer cells, and will provide new evidence for the anti-tumor effect of 1α,25(OH)2D3 through lncRNAs.