细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤的实验研究
Role of SOCS-1 on diabetic renal injury
目的：探讨细胞因子信号传导抑制蛋白1（Suppressors of cytokine signaling, SOCS-1）在糖尿病大鼠肾组织炎性损伤中的作用。方法：雄性CD-1小鼠随机分为四组，对照组、糖尿病组、SOCS-1质粒转染组和空载体转染组。腹腔注射链脲佐菌素（Streptozotocin, STZ）诱发糖尿病小鼠模型，转染组在成模后给予尾静脉注射SOCS-1质粒或空质粒载体，每隔7天注射一次。4周后收集标本，检测各组动物的血糖、24h尿蛋白；Western 蛋白印迹法检测肾组织中SOCS-1蛋白的水平。流式细胞术检测肾组织中巨噬细胞标志蛋白CD68的水平； ELISA检测肾组织中白细胞介素-1β（Interleukin-1β, IL-1β）和转化生长因子-β1（Transforming growth factor-β1, TGF-β1）的水平。结果：过表达SOCS-1能抑制糖尿病大鼠肾组织中CD68、IL-1β和TGF-β1的表达、降低糖尿病大鼠尿蛋白排泄，但对血糖无影响。结论：过表达SOCS-1能通过减轻糖尿病大鼠肾组织炎症反应从而缓解肾损伤。
Objective To investigate the role of SOCS-1 on diabetic renal injury. Methods Male CD-1 mice were randomly divided into four groups: control group, diabetic group, empty plasmid vector transfection group. SOCS-1 plasmid transfection group. Mice received an intraperitoneal injection of STZ at a dose of 150mgokg-1 body weight. Transfection mice received an injection of SOCS-1 plasmid or empty vector and every 7 days thereafter. At 4 weeks after STZ injection, specimens were collected to detect the blood glucose and 24 hours urine protein. Western blotting analysis were used to determine the expression of SOCS-1. The expression of CD68 was detected by flow cytometry. The secretion levels of IL-1β and TGF-β1 were detected by ELISA. Results Overexpression of SOCS-1 in kidney ameliorated excretion of urine protein and the expression of CD68, IL-1β and TGF-β1. Conclusion Overexpression of SOCS-1 can reduce the inflammatory response in renal tissue of diabetic rats and relieve renal injury.
刘淑霞、段惠军、邢玲玲、刘青娟
基础医学细胞生物学生理学
糖尿病肾病SOCS-1炎症IL-1β
kdiabetic nephropathySOCS-1inflammationIL-1β
刘淑霞,段惠军,邢玲玲,刘青娟.细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤的实验研究[EB/OL].(2012-03-16)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201203-505.点此复制
评论