胰岛素受体亚型在子宫内膜癌中的表达及作用初探

Objective: Hyperinsulinemia/diabetes in women is high-risk factors associated with endometrial carcinoma(EC), the molecular mechanism of which remains unclear. The aim of the study is to explore the expression and role of insulin receptor isoforms (IR-A and IR-B) in EC.Methods: The expression of IR isoforms were detected by RT-PCR and real-time PCR in four EC cells (Ishikawa、KLE、RL95-2、HEC-1-A) and EC tissues from 42 cases. The relationships between the expression of IR isoforms and the clinicopathological parameters of EC tissues were analyzed. An eukaryotic IR-A expression plasmid was constructed for overexpression of IR-A in RL95-2 cells, which originally has a low expression of IR-A. MTS method was used to determine the proliferation curves in RL95-2 cells with or without IR-A transfected.Results: IR-A and IR-B were co-expressed in four EC cells and EC tissues.The expression of IR-A and IR-B in EC tissues had no significant relevance with FIGO stage（P=0.838, P=0.146）, cell differentiation（P=0.556, P=0.272）, depth of myometrial invasion（P=0.440, P=0.206）, invasion of lympha-vascular space（P=0.115, P=0.104）and lymphonodes metastasis（P=0.864, P=0.166）.The expression of IR-A in EC patients with type 2 diabetes mellitus (DM) was significantly higher than that in patients without DM（P=0.043）.The overexpression of IR-A showed a significant proliferation-promoting effect on RL95-2 cells.Conclusions:The two isoforms of IR are co-expressed in EC, and the overexpression of IR-A may have the potential of promoting EC cell proliferation.