类固醇激素受体辅助调节因子ARA55和TGFβ1在前列腺癌细胞中作用分子机制的研究

Steroid hormone receptor (SHR) co-regulators (CoR) are the transcription factors recruited onto the promoter when steroid hormone binds to receptors, CoR may regulator target gene expression and activity in cells so that CoR may regulator cell proliferation, differentiation and apoptosis. In this paper we find that ARA55 expression decrease in prostate cancer cells and tissues. Overexpression of ARA55 can decrease proliferation of DU-145 cells. Real time RT-PCR and ELISA results show that overexpression of ARA55 can upregulate the expression of TGFβ1.TGFβ1 neutralizing antibody can partially block ARA55 desregulation of DU-145 proliferation. These results indicate ARA55 down regulates in DU-145 prostate cancer cell proliferation partially through TGFβ1. ARA55 expression decreased in prostate cancer. Overexpression of ARA55 inhibits DU-145 proliferation partially by upregulation of TGFβ1, while other pathways may be involved. These results uncover novel roles and mechanisms of ARA55 and TGFβ1 in the prostate cancer.