干扰素可诱导静息期CD4 T细胞中的APOBEC3G磷酸化

OBJECTIVE: To reveal the phosphorylation effect of interferon on APOBEC3G (A3G) in resting CD4 T cells. METHODS: A3G gene with HA-tag was amplified and cloned into eukaryotic expression vector pcDNA3.1 to construct plasmid pcDNA3.1-A3G-HA, and then, isolated CD4 T cells from human peripheral blood. The recombinant plasmid was transfected into resting CD4 T cells by nucleofector and the transfected cells were equally divided into two groups (control group and experimental groups). Then, the experimental group was treated by IFN-α. The phosphorylation of A3G in resting CD4 T cells was detected after co-immunoprecipitation (co-IP). RESULTS: Interferon can induce significant phosphorylation of A3g in resting CD4 T cells. CONCLUSIONS: Interferon induces the phosphorylation of A3G in resting CD4 T cells. This phenomenoncan indicates that IFN-α may be responsible for post-translation modification of A3G through some signal pathways and its molecular mechanism remains to be clarified.