LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization
LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization
Abstract Long non-coding RNAs (lncRNAs) play key roles in various biological processes. However, the roles of lncRNAs in macrophage polarization remain largely unexplored. In this study, thousands of lncRNAs were identified that are differentially expressed in distinct polarized bone marrow-derived macrophages (BMDMs). Among them, Dnmt3aos (DNA methyltransferase 3A, opposite strand), as a known lncRNA, locates on the antisense strand of Dnmt3a. Functional experiments further confirmed that Dnmt3aos were highly expressed in M(IL-4) macrophages and participated in the regulation of Dnmt3a expression, and played a key role in macrophage polarization. The DNA methylation profiles between the Dnmt3aos knockdown group and the control group in M(IL-4) macrophages were determined by MeDIP-seq technique for the first time, and the Dnmt3aos-Dnmt3a axis-mediated DNA methylation modification-regulated macrophage polarization related gene IFN-γ was identified. Our study will help to enrich our knowledge of the mechanism of macrophage polarization and will provide new insights for immunotherapy in macrophage-associated diseases.
Li Xueqin、Zhang Yingying、Pei Weiya、Zhu Xiaolong、Lv Kun、Yang Hui、Xu Yang、Kong Xiang、Zhong Min、Zhang Mengying、Chen Tianbing、Ye Jingjing
Central Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeNon-coding RNA Research Center of Wannan Medical College||Laboratory Medicine of Yijishan Hospital, Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College||Non-coding RNA Research Center of Wannan Medical CollegeCentral Laboratory of Yijishan Hospital, Wannan Medical College
基础医学生物科学研究方法、生物科学研究技术分子生物学
LncRNADNA methylationDnmt3amacrophage polarization
Li Xueqin,Zhang Yingying,Pei Weiya,Zhu Xiaolong,Lv Kun,Yang Hui,Xu Yang,Kong Xiang,Zhong Min,Zhang Mengying,Chen Tianbing,Ye Jingjing.LncRNA Dnmt3aos regulates Dnmt3a expression leading to aberrant DNA methylation in macrophage polarization[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/514307.点此复制
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