基于连通区域计数法对活体粘连细胞计数

ue to the possibility of cells adhesion, overlap and other situations in the living cells culture environment, an improved counting method based on the connected domain for color living adherent cell images were proposed. According to the morphological characteristics of cells, a combination method with the opening and closing operation and connected domain filling was used in the image preprocessing procedure. Then, the watershed transform with distance transformation was presented to realize the cells segmentation. Finally, the improved counting method based on the connected domain was proposed and analyzed to precisely calculate the quantity of cells. The experimental results show that the identification method works well with the advantages of accurately, high speed and good robustness.