蒙古马酪氨酸关联蛋白1（TYRP1）基因多态性与毛色表型相关性研究

In order to discuss the relationship between TYRP1 gene and phenotype of horse's coat color. In the study, the TYRP1 gene polymorphism were detected by PCR-SSCP in 3 breeds which included the Mongolian horse (5 groups), Sanhe horse and Thoroughbred ,the relative expression quantity in different colors skin of Mongolian horse were analyzed by Real-time quantitative PCR. The correlation between the polymorphism and coat color trait were analyzed. The results showed that the TYRP1 gene of Inner Mongolian horse has a missense mutation (C→T) in exon 2 and two genotypes B/B and B/Bb were detected. According to the analyzed results of population genetics, The expression level of TYRP1 gene was highest in chestnut skin and lowest in gray skin was detected by Real-time quantitative PCR. The results indicated that the TYPR1 gene is mainly related with colour deep or light and coloured coat formation.