Analysis of mutations in West Australian populations of Blumeria graminis f. sp. hordei CYP51 conferring resistance to DMI fungicides
Analysis of mutations in West Australian populations of Blumeria graminis f. sp. hordei CYP51 conferring resistance to DMI fungicides
Summary Powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) is a constant threat to barley production but is generally well controlled through combinations of host genetics and fungicides. An epidemic of barley powdery mildew was observed from 2007 to 2013 in the West Australian wheatbelt (WA). We collected isolates, examined their sensitivity to demethylation inhibitor (DMI) fungicides and sequenced the Cyp51B target gene. Five amino acid substitutions were found of which four were novel. A clear association was established between combinations of mutations and altered levels of resistance to DMIs. The most resistant genotypes increased in prevalence from 0 in 2009 to 16% in 2010 and 90% in 2011. Yeast strains expressing the Bgh Cyp51 genotypes replicated the altered sensitivity to various DMIs and these results were confirmed by in silico protein docking studies.
Lopez-Ruiz F.、Mullins J. G. L.、Jayasena K.、Oliver R. P.、Tucker M. A.、Cools H. J.
Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin UniversityInstitute of Life Science and College of Medicine, Swansea UniversityDepartment of Primary Research and Industry DevelopmentCentre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin UniversityCentre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin UniversityBiological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom, current address Syngenta, Jealott?ˉs Hill International Research Centre
植物保护遗传学微生物学
TriazolesDMIfungicideBlumeria graminis f. sp. hordei (barley powdery mildew)CYP51cross resistance
Lopez-Ruiz F.,Mullins J. G. L.,Jayasena K.,Oliver R. P.,Tucker M. A.,Cools H. J..Analysis of mutations in West Australian populations of Blumeria graminis f. sp. hordei CYP51 conferring resistance to DMI fungicides[EB/OL].(2025-03-28)[2025-08-11].https://www.biorxiv.org/content/10.1101/696906.点此复制
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