水稻传统品种月亮谷抗感品系与稻瘟病菌的群体互作

Population genetic characteristic of 540 M. oryzae isolates, pathogenic to rice Hexi 22-2, landrace Acuce pure lines with susceptible or resistant phenotype and natural population, were analyzed by using 15 SSR markers. The results revealed that all the isolates distinctly diversified into individual lineage (Ht=0.15±0.13，I=0.27±0.19, Fst=0.30), significant variation of inter- varietal and lines strains was observed(p<0.05). With cluster analysis, at similarity coefficient 0.9 level, there are only 1 dominant lineages in the isolates population from resistant Hexi 22-2 and resistant Acuce pure lines, however, 4-8 lineages was observed on blast-susceptible lines. This result indicated that resistant modern variety and blast-resistant Acuce pure lines exert selection pressure on M. oryzae population evolution. Further, for clarifying isolates diversification on single lesions, the isolates from single lesion of individual material was analyzed through clustering method, ten isolates from each panicle was clustered into 1-5 lineages, it impled that one colonization system could consist of isolates with sophisticated genetic source. AMOVA result based on 540 isolates analysis showed that intra-population variance contributed 78% variance to the population genetic variance of M. oryzae