玻璃化法处理的同种异体动脉移植的实验研究

Objective To compare vitrification and freezing to preserve the traditional treatment of allograft vascular effects after transplantation, and to find a more practical and easy production of vascular treatment methods.Methods Health bruising blue rabbit 96, surgical resection of bilateral femoral artery, according to different methods of in vitro vascular preconditioning experiment were divided into 3 groups: A new vascular autograft; B irradiation pretreatment frozen allograft vascular; C glass+irradiation pretreatment of vascular allografts. Vascular grafts after 1 week, 4 weeks, 8 weeks, 12 weeks, each taking six experimental animals for abdominal aortic digital subtraction angiography, scanning electron microscopy, histopathology and other methods to observe the vascular graft is usually the rate of aneurysm formation Morphological changes and organization.Results The results of 12 weeks after transplantation, A group the cumulative patency rate of 91.67%, B group was 70.83%, C group was 79.2%, A group and C group, B group and C group, P> 0.05, difference not statistically significant. A group and B group, P <0.05 difference has statistical significance. Histopathological examination revealed, C group treatment of allograft vascular smooth muscle proliferative endometrium and the middle group than the B light, the lumen stenosis was not obvious, less inflammatory response. Conclusion The vitrification irradiation preservation of allograft blood vessels, making the procedure easy, high patency rate of grafts, tissue reaction to light, are an ideal way to deal with allogenic blood vessels in vitro.