不同驯化菌群降解乙苯的性能及其生物群落结构研究

o gain insight into relationship between biodegradative characteristics and microbial community structure of different acclimated bacteria in degrading ethylbenzene, bacteria acclimated by benzene, toluene, ethylbenzene, xylene (BTEX) and BTEX mixture were used to degrade ethylbenzene, then degradation characteristics was explored and bacterial diversity was investigated by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis). Genomic DNA of bacteria was extracted after cultured by LB medium, 16S rRNA fragments were amplified with universal primers (F357-GC and R518) and analyzed by DGGE. Experimental results demonstrate that five kinds of acclimated bacteria had good effect on degrading ethylbenzene, in 54 h all degradation ratios reached 80% or more. Ethylbenzene domesticated bacteria had the best degradation efficient. There were some differences of dominant bacteria and community structure in different degradation stages under different acclimation. The PCR-DGGE method was proved to be a good tool for analysis of microbial community structure to optimize BTEX-degrading bacteria.