Cell atlas of the regenerating human liver after portal vein embolization
Cell atlas of the regenerating human liver after portal vein embolization
The liver has the remarkable capacity to regenerate. In the clinic, this capacity can be induced by portal vein embolization (PVE), which redirects portal blood flow resulting in liver hypertrophy in locations with increased blood supply, and atrophy of embolized segments. Here we apply single-cell and single-nucleus transcriptomics on healthy, hypertrophied, and atrophied patient-derived liver samples to explore cell states in the liver during regeneration. We first establish an atlas of cell subtypes from the healthy human liver using fresh and frozen tissues, and then compare post-PVE samples with their reference counterparts. We find that PVE alters portal-central zonation of hepatocytes and endothelial cells. Embolization upregulates expression programs associated with development, cellular adhesion and inflammation across cell types. Analysis of interlineage crosstalk revealed key roles for immune cells in modulating regenerating tissue responses. Altogether, our data provides a rich resource for understanding homeostatic mechanisms arising during human liver regeneration and degeneration.
Treutlein Barbara、Schaffer Theresa、Eckel Julian Connor、Santel Malgorzata、Hampe Jochen、Damm Georg、Camp J. Gray、Denecke Timm、Brazovskaja Agnieska、Brosch Mario、H?nsel Ren¨|、He Zhisong、K?rner Christiane、Gomes Tom¨￠s、Dannemann Michael、Seehofer Daniel
Max Planck Institute for Evolutionary Anthropology||Department of Biosystems Science and EngineeringMax Planck Institute for Evolutionary AnthropologyDepartment of Hepatobiliary Surgery and Visceral Transplantation, Leipzig UniversityDepartment of Biosystems Science and EngineeringMedical Department 1, University Hospital Dresden, Technical University Dresden||Center for Regenerative Therapies Dresden (CRTD), Technische Universit?t Dresden (TU Dresden)Department of Hepatobiliary Surgery and Visceral Transplantation, Leipzig UniversityMax Planck Institute for Evolutionary Anthropology||Institute of Molecular and Clinical Ophthalmology Basel||Department of Ophthalmology, University of BaselDepartment of Diagnostic and Interventional Radiology, Leipzig UniversityMax Planck Institute for Evolutionary AnthropologyMedical Department 1, University Hospital Dresden, Technical University Dresden||Center for Regenerative Therapies Dresden (CRTD), Technische Universit?t Dresden (TU Dresden)Department of Hepatobiliary Surgery and Visceral Transplantation, Leipzig University||Institute for Medical Informatics, Statistics and Epidemiology (IMISE), Leipzig UniversityDepartment of Biosystems Science and EngineeringDepartment of Hepatobiliary Surgery and Visceral Transplantation, Leipzig University||Rudolf-Sch?nheimer Institute for Biochemistry, Faculty of Medicine, Leipzig UniversityDepartment of Biosystems Science and EngineeringMax Planck Institute for Evolutionary Anthropology||Institute of Genomics, University of TartuDepartment of Hepatobiliary Surgery and Visceral Transplantation, Leipzig University
基础医学细胞生物学分子生物学
liverscRNA-seqregenerationhumanportal vein embolization
Treutlein Barbara,Schaffer Theresa,Eckel Julian Connor,Santel Malgorzata,Hampe Jochen,Damm Georg,Camp J. Gray,Denecke Timm,Brazovskaja Agnieska,Brosch Mario,H?nsel Ren¨|,He Zhisong,K?rner Christiane,Gomes Tom¨￠s,Dannemann Michael,Seehofer Daniel.Cell atlas of the regenerating human liver after portal vein embolization[EB/OL].(2025-03-28)[2025-05-17].https://www.biorxiv.org/content/10.1101/2021.06.03.444016.点此复制
评论