低温等离子体诱变镍吸附细菌B8

Radio frequency low temperature plasma was used to mutate the strain B8 which can adsorb nickel ions, and the adsorbability of the mutant was tested. The experiments showed that the optimum mutagenic conditions of B8 by plasma were a carrier gas of N2, a time of 3 min, a power of 50-65 W, a carrier gas pressure of 20 Pa. The high adsorption mutant Ni12 was screened from the mutant strains under that conditions, and the adsorption on nickel ions of the mutant Ni12 reached 136.7 mg/g (dry bacteria), which was 11.7% higher than that of wild strain B8. The genetic stability of mutant Ni12 maintained in 5 generations. After the formation of biofilm on the porous ceramics, the adsorption rate of Ni12 on the nickel ion solution (the concentration was 50 mg/L) reached 86%. The result of 16S rDNA sequence analysis indicated that the mutant Ni12 was Pseudomonas cedrina. The advantages of mutagenesis by radio frequency low temperature plasma on wild strain B8 were high efficiency, and the mutant strain Ni12 was stable inheritance and promising in treatment of wastewater for the strong adsorption on nickel ions.