发光杆菌中新型双元毒素对根结线虫活性测定

Photorhabuds luminescens is a facultative anaerobic bacteria belonging to the family of Enterobacteriaceae. Upon entering an insect host, nematodes release the bacteria by regurgitation directly into the insect's hemocoel. The bacteria kill the insect host by producing a number of toxins. With the completion of the genome sequencing, various functional proteins have been identified and characterized from P. luminescens. New binary toxins Plu1961 and Plu1962 were identified from this bacterial. In this study, the nematicidal activities of the binary toxins against Meloidogyne spp. were assayed. The data showed that all the two toxin shows obvious activity two second-stage juvenile, and the combination of the two toxin showed more high toxicity, which suggested that the binary toxins exhibit obviously synergetic activity. The results provided a new way for the biological control of nematodes, supplied a new source for the development of new biological control agents.