逍遥散对慢性温和不可预知应激模型大鼠的影响

Objective: The study the effect of Xiaoyao San on changes of behavior and monoamine neurotransmitters concentration in chronic unpredictable mild stress (CUMS) model in rats. Methods: The stimulus factors of CUMS model lasted for 11 weeks. From the 4th week, the rats were treated with the decoction of Xiaoyao San (19.5g·kg-1 or 25.0g·kg-1, ig) or imipramine (10mg·kg-1, ip) according to their groups for 8 weeks. During the experiment, body weight, consumption of sucrose solution, and data of open- field test （including numbers of crossing and standings）were detected, which was to evaluate the anti- depression action of Xiaoyao San. In the 11th week, all rats were decapitated 30min after the last treatment, then prefrontal cortex and hippocampus were immediately isolated for detecting the concentration of 5-HIAA, 5-HT, DA and NE by fluorospectrophotometry. Results: After 3 weeks of CUMS procedure, sucrose consumption, crossing and standing of model group decreased significantly (compared with control group, P<0.01). Continually administrated for 2 weeks, Xiaoyao San (19.5g·kg-1 and 25.0g·kg-1) increased sucrose consumption significantly. However，continuous treatment of Xiaoyao San for 4 weeks and 7 weeks, the effect of increasing sucrose consumption was not significant compared with model group, while XYS (25.0g·kg-1) showed increasing trend on weight, the number of crossing and standing in rats. Continuous treatment of Xiaoyao san (25.0g·kg-1) for 8 weeks, could increase the concentration of 5-HT in prefrontal cortex and 5-HIAA in hippocampus (P<0.01, P<0.05). Conclusions: It suggested that Xiaoyao San had the anti- depression effect, which the mechanism was possibly related to affecting the brain monoamine neurotransmitters level, especially 5-HT.