植物乳杆菌CCFM420抑制结肠癌发生的机制研究

olon cancer is a serious threat to the health of chinese people with the increase in morbidity. In addition to the traditional treatment, the use of probiotics to prevent and alleviate the occurrence of colon cancer may be an effective intervention. [Objective] construct an in vitro model of Lactobacillus plantarum with inhibitory effect on colon cancer. base on the experimental results of animal model. Explore the mechanism of L. plantarum to alleviate the occurrence of colon cancer. [Methods] The effect of L. plantarum on the growth of colon cancer cells and the effects on the signal pathway of colon cancer were evaluated with HT-29 cell model. Strains with the potential of inhibiting colon cancer was obtained. The colorectal cancer mice were used as animal models. L. rhamnosus GG strain and Escherichia coli were used as positive and negative control. The number of colorectal cancer cells, serum cytokine concentration, colorectal cancer-related signal pathway and fecal Short chain fatty acid concentration was evaluated to confirm the ability of L. plantarum to inhibit colon cancer in vivo. [Results] L. plantarum CCFM420 has a good ability to inhibit colon cancer cell proliferation and regulate cancer-related signaling pathway. This strain significantly reduced the number of colon cancer mice, reduced the body's inflammatory response and regulated the body's cancer-related pathways. [Conclusion] CCFM420 has the potential to inhibit the occurrence of colon cancer. This strain may down regulated the Notch signaling pathway by reducing the intestinal levels of butyric acid, thereby inhibiting the occurrence of colon cancer.