小鼠1号染色体Adam23基因的印记分析

Imprinted genes play important roles in fetal growth, development and maternal behavior in mammals. The correct dosage of imprinted genes is implicated in abnormalities of development and many diseases including cancer. Therefore, it is necessary to research genomic imprinting and identify novel imprinted genes for not only the developments of developmental genetics, also the study of mechanisms of related diseases. The main task of the study of genomic imprinting is the characterize of new imprinted genes. In the paper, imprinting status of the Adam23 gege in Zdbf2-Gpr1 imprinting domain on chromosome 1 were analysed as candidate imprinted genes based on clusters features of imprinted genes by direct sequencing of SNPs approach. The results of imprinting analysis showed that the gene was preferential paternally expressed in the mouse. That is, Adam23 was a gene of incomplete imprinting and characteristic of the genes located on the terminal of imprinting domain.