高度活化的子宫NK细胞在小鼠妊娠过程中起着关键性调节作用

o understand the response of murine uterine natural killer (uNK) cells to Toll-like receptor (TLR) 3 agonist at the early stage of gestation, CBA×DBA/2 mice were intraperitoneally (i.p.) injected with polyinosinic-polycytidylic acid (poly I:C), the specific TLR-3 agonist, at dose of 10 μg/g·BW or PBS at gestation day (gd) 6.5. The percentage of intracellular TNF-α+ or IFN-γ+ uNK (DX5+CD3-) cells in the implantation sites of CBA×DBA/2 matings were significantly increased 24 hours after poly I:C injection. Surprisingly, poly I:C treatment significantly decreased the total number of uNK cells (either DX5+CD3- or DBA+) at fetal maternal surface, but had no influence on local NKT cells, T cells and DCs. This investigation will help to explain the central role for hyperactivated uNK cells in the progress of mice pregnancy.