结核分枝杆菌ABC转运器胞外结构域COG1732的结晶研究

purpose] COG1732 protein is one of the ATP-binding cassette substract binding protein for Mycobacterium tuberculosis H37Rv.unfortunatly. COG1732 protein three-dimension structure and fuction is not conformed,bioinformation analysis cue COG1732 protein maybe participate in osmosis uptake into cell and identify substance.then change cross membrane structure.Purified COG1732 protein for crystall and determined [method]For ABC transporter substrate binding dCrystallization for the outer membrane subunit COG1732 of Mycobacterium tuberculosis H37Rvomain COG1732 oCrystallization for the outer membrane subunit COG1732 of Mycobacterium tuberculosisf Mycobacterium tuberculosis H37Rv use heterologoCrystallization for the outer membrane subunit COG1732 of Mycobacterium tuberculosis H37Rvus expression,constructed the COG1732 to pet28b with T7 promoter .and over-expression COG1732 in E.coli BL21(DE3).Target protein was purified by NI affinity chromatography and ions exchange chromatography.Purified COG1731 protein concentrated to 70mg/ml,Partial and store at -80 freezer. At the same time for crystall screen.[result]Get crystll for target protein COG1732 with hangle vapor diffusion mothed [conclusion]We have successfully constructed COG1732 protein prokaryotic exprisson vector,And established a prefect purification protocol of recombinant protein and get the crystall condition ,established the foundtion for resolved its structure.