慢性粒细胞白血病ZO-1基因启动子区甲基化状态分析及临床意义初步探讨

Objective To investigate the methylation status of Zonula occludens 1(ZO-1) gene promoter in the chronic and blastic phase CML patients’ bone marrows，discuss its clinic sense in the pathogenesis and progression of chronic myelocytic leukemia. Methods The methylation pattern in promoter region of ZO-1 gene was detected with methylation specific PCR (MS-PCR) in bone marrow samples of 10 chronic phase CML patients and 10 blastic phase CML patientss .Results (1) The promoter region of ZO-1 gene was unmethylated in 10 cases of chronic phase patients；otherwise the methylation frequency of ZO-1 gene promoter region in 10 cases of CML-BP was 70% (7/10).(2) For one CML patient who progressed to BP and got back to CP after treatment, the promoter region of ZO-1 gene was unmethylated in chronic phase and methylated in blastic phase, otherwise, the methylation pattern of ZO-1 was trans back to unmethylated when the disease returned to the chronic phase. Conclusion The hypermethylated status of ZO-1 gene promoter region was specificitly detected in human CML blastic phase, it was closely correlated with the progression of the disease and might be a molecular maker which can prognosticate the progression to BP.