UCH-L3在小鼠腹水型肝癌高、低淋巴道转移株中的表达

BACKGTOUND & OBJECTIVE: Most of patients with cancer died from its metastasis, the mechanism of metastasis and prevention and therapeutics have been an important issue in tumor domain. Hca-F(highly lymphatic metastasis potential) and Hca-P(low metastasis) are a pair of syngeneic mouse hepatocellular cell lines which present different potential of lymphatic metastasis. UCH-L3 belongs to the ubiquitin C-terminal hydrolase family. There are little reports concerning the role of UCH-L3 in tumor, especially regarding its correlation with tumor metastasis. In our study, the expression of UCH-L3 were detected in Hca-F and Hca-P and analyzed the relationship between the two tumor cell lines in order to explore the role of encoding protein of UCH-L3 genes in tumor metastasis and its relevant molecular mechanisms. METHODS: the expression of UCH-L3 protein were detected in Hca-F and Hca-P tumor cells by immunocytochemistry, Western Blot and flow cytometry. RESULTS: Immunocytochemistry manifests that UCH-L3 was localized to the cytosol between the two tumor cells. Western Blot and flow cytometry indicate that the expression of UCH-L3 was lower in Hca-F than in Hca-P (P<0.01, P<0.001) .CONCLUSION: The expression of UCH-L3 may be related to the lymphatic metastasis.