黔产剑叶凤尾蕨的化学成分研究

o study the chemical const ituents of Pteris ensiformis ensiformis. In this paper, silica gel, gel, MCI, C C18 and other column chromatography for separation and purification purification, and their structures were identified by 1H-NMR, 13 C-NMR, MS and IR spectra ; the antianti-tumor and anti anti-coagulation activiti es of some monomers were screened by MTS, APTT, PT and TT. The results were as follows follows( 15 compounds were isolated from the P. ensiformis , the compounds were 2 2-hydroxylhydroxyl-acetyl pyrrole ( 1), N N-(3 - carboxypropyl)carboxypropyl)-2-acetyl pyrrole ( 2), 3 3-hydroxylhydroxyl-2-methylpyr idine ( 3), N N-methyl hydroxylamine ( 4), pterosin S 13 13-O-glucoside ( 5), obtupterosin C 6), entent-11 α- hydroxyhydroxy-15 -oxokauranoxokauran-19 -oic acid ( 7), entent-11 α-hydroxyhydroxy-15 -oxokauroxokaur-16 -en -19 -oic acid ( 8), β-sitosterol ( 9), entent-11 α-hydroxyhydroxy-15 -oxooxo-kaurkaur-16 -en -19 -oic acid acid-O-glu copy ranoside ( 10 ), 5, 5'-dibutoxydibutoxy-2, 2' 2'-bifuran ( 11 ), 5, 5'-di(2 -ethylethyl-hexyloxy)hexyloxy)-2, 2' 2'-bifuran (12 ), (-)-loliolide ( 13 ), succinic acid 14 ), fumaric acid 15 ). Compound 1 is a new natural product of pyrrole alkaloids. Compounds 1-7, 10 -15 were isolated fr from P. ensiformis for the first time, and compounds 1, 3, 4 were isolated from Pteris for the first time; (2) The results of activity testshowed that compounds 1, 2, 3, 5, 6 and 10 inhibited the growth of tumor cells HL HL-60, A549, SMMC SMMC-7721, MDA MDA-MB -231 and SW480 in vitro at a concentration of 40 μmol/L , At the concentration of 2.0 L, compounds 1, 2, 3 and 6 shortened APTT and compounds 1, 5 and 6 prolonged PT. The study enrichs the chemical constituent of Guizhou P. ensiformis and provide a material basi s for the development of anti anti-tumor drugs.