Selective Cell Propagation via Micropatterning of Thermal-activated Hydrogel
Selective Cell Propagation via Micropatterning of Thermal-activated Hydrogel
Abstract The ability to selectively propagate specific cells is fundamentally important to the development of clonal cell populations. Current methods rely on techniques such as limiting dilution, colony picking, and flow cytometry to transfer single cells into single wells, resulting in workflows that are low-throughput, slowed by propagation kinetics, and susceptible to contamination. Here, we developed a method, called selective laser gelation (SLG), to micropattern hydrogels in cell culture media in order to encapsulate specific cells to selectively arrest their growth. This process relies on the inverse gelation of methylcellulose, which forms a hydrogel when heated rather than cooled. Local heating using an infrared laser enables hydrogel micropatterning, while phase transition hysteresis retains the hydrogel after laser excitation. As a demonstration, we used this approach to selectively propagate transgenic CHO cells with increased antibody productivity. More generally, hydrogel micropatterning provides a simple and non-contact method to selective propagation of cells based on features identified by imaging. One Sentence SummaryInverse gelation of methylcellulose enables hydrogel micropatterning to selectively propagate cells identified by microscopy.
Duffy Simon P.、Ma Hongshen、Teodoro Joyce A.、Chiu Jeffrey C.Y.、Lee Jeong Hyun、Matthews Kerryn
Department of Mechanical Engineering, University of British Columbia||Centre for Blood Research, University of British ColumbiaDepartment of Mechanical Engineering, University of British Columbia||Centre for Blood Research, University of British Columbia||School of Biomedical Engineering, University of British Columbia||Vancouver Prostate Centre, Vancouver General HospitalDepartment of Mechanical Engineering, University of British Columbia||Centre for Blood Research, University of British ColumbiaDepartment of Mechanical Engineering, University of British Columbia||Centre for Blood Research, University of British ColumbiaDepartment of Mechanical Engineering, University of British Columbia||Centre for Blood Research, University of British ColumbiaDepartment of Mechanical Engineering, University of British Columbia||Centre for Blood Research, University of British Columbia
生物科学研究方法、生物科学研究技术细胞生物学生物工程学
HydrogelMicropatterningMethylcelluloseScreening
Duffy Simon P.,Ma Hongshen,Teodoro Joyce A.,Chiu Jeffrey C.Y.,Lee Jeong Hyun,Matthews Kerryn.Selective Cell Propagation via Micropatterning of Thermal-activated Hydrogel[EB/OL].(2025-03-28)[2025-06-19].https://www.biorxiv.org/content/10.1101/2020.04.01.019208.点此复制
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