没食子儿茶素没食子酸酯降低P糖蛋白表达逆转人耐药鼻咽癌细胞株多药耐药的研究

Objective To evaluate the value of （-）epigallocatechin-3-gallate (EGCG) on human nasopharyngeal carcinoma HNE1/DDP cell lines and its possible mechanisms. Methods Cytotoxicity of EGCG and its sensibility to Cis-platinum(DDP) in HNE1/DDP cell line, were evaluated by Methyl thiazolyl tetrazolium (MTT) assay . Before and after EGCG was given, the expressions of P- glycoprotein were detected by Quantitative real-time polymerase chain reaction (QRT-PCR) and Western blot respectively. Results The 10% inhibition concentration (IC10) of EGCG to HNE1/DDP cell line was 154.6umol/L, so 60umol/L,80umol/L and 120umol/L of EGCG were used to detect the reversal effect of multidrug resistance. IC50 for DDP was 4.22ug/mL .EGCG could increase the drug susceptibility of HNE1/DDP cell to DDP, and at the concentrations of those above .The IC50 for DDP decreased to3.82ug/mL, 2.56ug/mL and 1.78ug/mL, and the modulating factors were 1.10，1.65 and 2.37 fold respectively. The expressions of P-glycoprotein in HNE1/DDP cells incubated with EGCG were deceased when detected by QRT-PCR and Western blot. Conclusion EGCG could partially reverse the multidrug resistance of the HNE1/DDP cells in vitro. Moreover, the reversal effect may be due to the increase of intracellular accumulation of chemotherapeutic drugs and the decrease of the expression of P-glycoprotein .