锰过氧化物酶的酵母重组表达及其降解黄曲霉毒素B1研究

In this study, food-grade recombinant Kluyveromyces lactis strains GG799 (pKLAC1-Phsmnp), GG799 (pKLAC1-Plomnp), and GG799 (pKLAC1-Phcmnp) were constructed.Heterologous expression of three different sources of manganese peroxidase in Kluyveromyces lactis was achieved, and the degradation effects of three recombinant Kluyveromyces lactis fermentation supernatants on aflatoxin B1 were compared.It was found that GG799 (pKLAC1-Phcmnp) had the best degradation effect on aflatoxin B1, reaching 50.52%.This study also optimized the inducible expression conditions of the recombinant strain GG799 (pKLAC1-Phcmnp). Under the optimal induction conditions optimized by orthogonal experiments, the degradation rate of AFB1 by the recombinant fermentation supernatant could reach 67.4%. Compared with the previous one, the degradation rate was increased by 16.88%, and compared with the single factor optimization, the degradation rate was increased by 9.08%.