Post-embryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
Post-embryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
Abstract BackgroundThe axolotl is a key model to study appendicular regeneration. The limb complexity resembles that of humans in structure and tissue components; however, axolotl limbs develop post-embryonically. In this work, we evaluated the post-embryonic development of the appendicular skeleton and its changes with aging. ResultsThe juvenile limb skeleton is formed mostly by Sox9/Col1a2 cartilage cells. Ossification of the appendicular skeleton starts when animals reach a length of 10 cm, and cartilage cells are replaced by a primary ossification center, consisting of cortical bone and an adipocyte-filled marrow cavity. Vascularization is associated with the ossification center and the marrow cavity formation. We identified the contribution of Col1a2-descendants to bone and adipocytes. Moreover, ossification progresses with age towards the epiphyses of long bones. Axolotls are neotenic salamanders, and still ossification remains responsive to L-thyroxine, increasing the rate of bone formation. ConclusionsIn axolotls, bone maturation is a continuous process that extends throughout their life. Ossification of the appendicular bones is slow and continues until the complete element is ossified. The cellular components of the appendicular skeleton change accordingly during ossification, creating a heterogenous landscape in each element. The continuous maturation of the bone is accompanied by a continuous body growth.
Riquelme-Guzm¨￠n Camilo、Schuez Maritta、Edwards-Jorquera Sandra、Knapp Dunja、Ceccarelli Alberto S.、Sandoval-Guzm¨￠n Tatiana、Rauner Martina、B?hm Alexander、Chara Osvaldo
Technische Universit?t Dresden, CRTD/ Center for Regenerative Therapies TU DresdenTechnische Universit?t Dresden, CRTD/ Center for Regenerative Therapies TU DresdenTechnische Universit?t Dresden, CRTD/ Center for Regenerative Therapies TU DresdenTechnische Universit?t Dresden, CRTD/ Center for Regenerative Therapies TU DresdenSystem Biology Group (SysBio), Institute of Physics of Liquids and Biological Systems (IFLySiB), National Scientific and Technical Research Council (CONICET) and University of La PlataTechnische Universit?t Dresden, CRTD/ Center for Regenerative Therapies TU Dresden||Center for Healthy Aging, Universit?tsklinikum DresdenDepartment of Medicine III, Universit?tsklinikum Dresden||Center for Healthy Aging, Universit?tsklinikum DresdenTechnische Universit?t Dresden, CRTD/ Center for Regenerative Therapies TU DresdenSystem Biology Group (SysBio), Institute of Physics of Liquids and Biological Systems (IFLySiB), National Scientific and Technical Research Council (CONICET) and University of La Plata||Instituto de Tecnolog¨aa, Universidad Argentina Empresa (UADE)||Center for Information Services and High Performance Computing (ZIH), Technische Universit?t Dresden
动物学细胞生物学生理学
Axolotlossificationagingchondrocytesosteoblasts
Riquelme-Guzm¨￠n Camilo,Schuez Maritta,Edwards-Jorquera Sandra,Knapp Dunja,Ceccarelli Alberto S.,Sandoval-Guzm¨￠n Tatiana,Rauner Martina,B?hm Alexander,Chara Osvaldo.Post-embryonic development and aging of the appendicular skeleton in Ambystoma mexicanum[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2021.03.05.434057.点此复制
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