PAK5与LMO2蛋白相互作用的初步研究
haracterization of the novel interaction between PAK5 and LMO2
目的：应用酵母双杂交技术筛选PAK5（p21 activated kinase 5）的相互作用蛋白。方法：利用酵母双杂交技术，以PAK5为诱饵，从人胎脑文库中寻找出与PAK5相互作用的蛋白，并用GST pull-down,免疫共沉淀及共聚焦激光扫描显微镜技术进行验证。结果：经过酵母双杂交筛选，获得阳性克隆，进一步测序分析，确定筛选出的相互作用蛋白-LOM2（LIM domain Only protein 2）。结论：PAK5新的相互作用蛋白LMO2的发现，为揭示PAK5发挥生物学功能的分子机制提供线索，有望为肿瘤治疗提供新的理论依据。
Objective:Screening of novel PAK5-interacting proteins by yeast two-hybrid system.Methods:Using PAK5 as a bait,a human embryonal brain cDNA library was screened.After discarding the false postive clones,the truly positive clones were sequenced and submitted to BLAST analysis.The interaction was confirmed by in vitro pull-down assay,immunoprecipitation and laser scanning confocal microscopy.Results:LMO2,the novel PAK5-interacting protein were confirmed.Conclusion:This work would help to study the mechanism of the pleiotropic effects of PAK5，which might be a potential therapeutic strategy for tumor.
耿楠希、李洋、李丰、柯强
基础医学分子生物学肿瘤学
肿瘤PAK5酵母双杂交LMO2
tumorPAK5yeast two-hybridLMO2
耿楠希,李洋,李丰,柯强.PAK5与LMO2蛋白相互作用的初步研究[EB/OL].(2013-12-04)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/201312-74.点此复制
评论