普通小麦-华山新麦草-黑麦三属杂种后代衍生系的细胞遗传学鉴定

Wheat relatives contain abundant germplasm resource, and trigeneric hybrids can reap the excellent wheat germplasm with the genes from the relative species and genus of Triticum. the trigeneric hybrid involving Triticum, Psathyrostachys and Secale was synthesized by crossing wheat-P. huashanica amphiploid (PHW-SA, 2n = 56, AABBDDNsNs) with wheat-S. cereale amphiploid (Zhongsi 828, 2n = 42, AABBRR). In this study, Meiotic analysis showed that the chromosomes number of the F4 progenies ranged 39 to 46, frequency of lines with 42 chromosomes was more than 50%. The average chromosome configuration at MI of pollen mother cells (PMCs) of trigeneric hybrids F4 was 2n = 1.71Ⅰ + 20.26Ⅱ + 0.04Ⅲ + 0.001Ⅳ, and multivalents consisted of trivalents and tetravalents can be observed in 52.7% of the plants. GISH results revealed that most F4 plants had 11-14 S. cereale chromosomes, and P. huashanica chromosome was undetected. So the trigeneric hybrid progenies could be lay a foundation for creating new germplasm resources.