FIB-SEM analysis on three-dimensional structures of growing organelles in wild Chlorella pyrenoidosa cells
FIB-SEM analysis on three-dimensional structures of growing organelles in wild Chlorella pyrenoidosa cells
Abstract To clarify dynamic changes of organelle microstructures in Chlorella pyrenoidosa cells during photosynthetic growth with CO2 fixation, three-dimensional organelle microstructures in three growth periods of meristem, elongation and maturity were quantitatively determined and comprehensively reconstructed with focused ion beam scanning electron microscopy (FIB-SEM). The single round-pancake mitochondria in each cell split into a dumbbell and then into a circular ring, while barycenter distance of mitochondria to chloroplast and nucleus was reduced to 45.5% and 88.3% to strengthen energy transfer, respectively. The single pyrenoid consisting of a large part and another small part in each chloroplast gradually developed to a mature state in which the two parts were nearly equal in size. The nucleolus progressively became larger with euchromatin replication. The number of starch grains gradually increased, but average grain volume remained nearly unchanged.
Chen Gongda、Guo Wangbiao、Guo Jiansheng、Zhang Xing、Chen Chuangting、Zou Xiangbo、Cheng Jun、Feng Lingchong、Qin Shiwei、Yang Weijuan、Rao Mumin、Ye Ji、Kuang Cao
Guangdong Energy Group Science and Technology Research Institute Co. Ltd.State Key Laboratory of Clean Energy Utilization, Zhejiang UniversityCenter of Cryo-Electron Microscopy, Zhejiang UniversityCenter of Cryo-Electron Microscopy, Zhejiang UniversityGuangdong Energy Group Science and Technology Research Institute Co. Ltd.Guangdong Energy Group Science and Technology Research Institute Co. Ltd.State Key Laboratory of Clean Energy Utilization, Zhejiang UniversityState Key Laboratory of Clean Energy Utilization, Zhejiang UniversityGuangdong Energy Group Science and Technology Research Institute Co. Ltd.State Key Laboratory of Clean Energy Utilization, Zhejiang UniversityGuangdong Energy Group Science and Technology Research Institute Co. Ltd.Guangdong Energy Group Science and Technology Research Institute Co. Ltd.Guangdong Energy Group Science and Technology Research Institute Co. Ltd.
细胞生物学植物学生物科学研究方法、生物科学研究技术
Chlorella pyrenoidosaHigh-pressure freezingFIB-SEM3D structureOrganelles
Chen Gongda,Guo Wangbiao,Guo Jiansheng,Zhang Xing,Chen Chuangting,Zou Xiangbo,Cheng Jun,Feng Lingchong,Qin Shiwei,Yang Weijuan,Rao Mumin,Ye Ji,Kuang Cao.FIB-SEM analysis on three-dimensional structures of growing organelles in wild Chlorella pyrenoidosa cells[EB/OL].(2025-03-28)[2025-05-04].https://www.biorxiv.org/content/10.1101/2022.05.08.491059.点此复制
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