竹节香附素A抑制人结肠癌细胞HCT-116迁移和侵袭的作用及机制研究

Objective: To investigate roles and mechanisms of RA on migration and invasion of human carcinoma colon HCT-116 cells. Methods: HCT-116 cells were treated with RA at concentration range of 0.125 to 50μM for 24h and 48h, respectively. Cell proliferation was assessed by MTT assay. Furthermore, cell migration at different concentrations of RA (0.25, 0.5 and 1μM) on HCT-116 cells was assayed by the scratch wound healing and Transwell chamber assay. And Matrigel cell invasion assay was used to detect the effect of RA on the invasion of HCT-116 cells．Real-time quantitative PCR and Western blotting were used to detect the mRNA and protein expressions of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in the cells, respectively. Results：Compared with the control, RA could significantly inhibited the proliferation and induced apoptosis to HCT-116 cells, when the concentration of RA is above 1μM. More important, we observed that RA (0.25, 0.5 and 1μM) below cytotoxic concentration significantly suppressed the metastatic activity of HCT-116, including wound healing, invasion and migration in concentration dependent manner. Further mechanistic studies revealed that RA treatment significantly reduced the gene and protein expression of MMP-2 and MMP-9 by Real-time PCR and Western blotting analysis. Conclusion: Our data, for the first time, demonstrate that RA can obviously inhibit the ability of HCT-116 cells proliferation, migration and invasion. In addition, the anti-metastatic effects of RA might be related to down-regulating the expression of gene and protein of MMP-2 and MMP-9.